Tryptic Soy Broth (TSB), USP, 100ml, 4oz glass bottle, Hardy Diagnostics
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U42 Tryptic Soy Broth (TSB), USP, 100ml, 4oz glass bottle 20 Pack
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For the cultivation of microorganisms. Also known as TSB or Soybean-Casein Digest Broth, USP.

Hardy Diagnostics Tryptic Soy Broth is recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of bacteria and fungi. The U.S. Pharmacopeia National Formulary (USP) describes its use for sterility testing.

This product is not intended to be used for the diagnosis of human disease.

Tryptic Soy Broth is widely used for the cultivation of microorganisms from environmental sources; supporting the growth of the majority of bacteria and fungi. Tubes of this medium may be used for preparing dilutions of organism for colony counts and preparation of standard inocula. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria.

Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Pharmacopeia. This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. Sodium chloride is added to maintain the osmotic equilibrium. Dextrose is incorporated as an energy source. The dipotassium phosphate is included in the formulation as a buffer to maintain the pH.


Ingredients per liter of deionized water:

  • Pancreatic Digest of Casein: 17.0gm
  • Sodium Chloride: 5.0gm
  • Papaic Digest of Soybean Meal: 3.0gm
  • Dextrose: 2.5gm
  • Dipotassium Phosphate: 2.5gm
  • Final pH: 7.3 +/- 0.2 at 25°C.

Storage and Shelf Life

Storage: Upon receipt store at 2-25°C. away from direct light. Media should not be used if there are any signs of deterioration (discoloration), contamination, or if the expiration date has passed. Protect from light, excessive heat, moisture, and freezing. The expiration dating on the product label applies to the product in its intact packaging when stored as directed. The product may be used and tested up to the expiration date on the product label and incubated for the recommended quality control incubation times. Hardy Diagnostics' sterility media and rinse solutions are manufactured in an ISO 13485 environmentally controlled production center. Each lot is certified for pH, sterility and growth promotion using ATCC® strains specified by the USP. Our approach to manufacturing ensures all sterility media and rinse fluids provide the highest level of clarity for improved reading accuracy, thus significantly reducing the risk of incorrect interpretation and false results.

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